Part:BBa_K092400:Design

LuxI with RBS and Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This biobrick is an alternative to the F1610 which didn't work.

This biobrick was done by 2-steps PCR, for more information go to visit our Wiki page on this subject : [http://2008.igem.org/Team:EPF-Lausanne/2-step_PCR EPF-Lausanne : 2-steps PCR]

Source

Source of LuxI : Plasmid pSND1 from Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4